Harvest of the mesocosms started 14 days after herbicide application by cutting the
remaining or untreated plants at the soil surface to obtain aboveground plant biomass
production. Afterwards, soil was removed from themesocosms in three separate layers
0–5 cm, 5–10 cm and 10–30 cm. Earthworms present in these soil layers were carefully
washed free of soil, placed onmoist filter paper, counted, weighed and released to
the BOKU garden. Roots present in these soil layers were washed free of attached soil
particles under a jet of tap water over a 1-mm sieve and sorted out. Dry mass of shoots
and roots was determined after 48 hours oven-drying at 55uC. A portion of roots per
layer was collected, cleared with boiling KOH for four minutes and stained for one
minute with black Sheaffer ink79. Percentage of root length colonized by AMF considering
arbuscules and vesicles (i.e. mycorrhization rate) was determined using the
grid-line method by counting at least 100 intersections per sample80.
Harvest of the mesocosms started 14 days after herbicide application by cutting the
remaining or untreated plants at the soil surface to obtain aboveground plant biomass
production. Afterwards, soil was removed from themesocosms in three separate layers
0–5 cm, 5–10 cm and 10–30 cm. Earthworms present in these soil layers were carefully
washed free of soil, placed onmoist filter paper, counted, weighed and released to
the BOKU garden. Roots present in these soil layers were washed free of attached soil
particles under a jet of tap water over a 1-mm sieve and sorted out. Dry mass of shoots
and roots was determined after 48 hours oven-drying at 55uC. A portion of roots per
layer was collected, cleared with boiling KOH for four minutes and stained for one
minute with black Sheaffer ink79. Percentage of root length colonized by AMF considering
arbuscules and vesicles (i.e. mycorrhization rate) was determined using the
grid-line method by counting at least 100 intersections per sample80.
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