Solubility and swelling tests of the flms concluded that the lignin incorporation reduced water afnity of
flm. Lignin incorporation produces a noticeable plasticizing effect on the blended flm, showing optimum values for
flm incorporated with 20 and 30 % (w/v) lignin, as deduced from their mechanical and thermal properties. Lignin
blended flm had lower glass transition temperatures (Tg) as compared to control gelatin. Infrared spectroscopy
(FTIR) analysis of flms suggested that lignin interacts with gelatin by hydrogen bonding and hydrophobic interaction
consequently creating conformational changes. Atomic force microscopic (AFM) study displays smooth surface of
synthesized flms. Light barrier properties of flm revealed that the lignin addition improved barrier properties against
UV light in the range of 280–350 nm. Furthermore, the lowest scavenging activity was observed in LG-E (111.10 µg/
ml) trailed by LG-D (249.29 µg/ml) and LG-C (259.53 µg/ml).