Vitamin C is one of the most important vitamins, so reliable information about its content in foodstuffs is
a concern to both consumers and quality control agencies. However, the heterogeneity of food matrixes
and the potential degradation of this vitamin during its analysis create enormous challenges. This review
addresses the development and validation of high-performance liquid chromatography methods for vitamin
C analysis in food commodities, during the period 2000–2014. The main characteristics of vitamin C
are mentioned, along with the strategies adopted by most authors during sample preparation (freezing
and acidification)to avoid vitamin oxidation. After that,the advantages and handicaps of different analytical
methods are discussed. Finally, the main aspects concerning method validation for vitamin C analysis
are critically discussed. Parameters such as selectivity, linearity, limit of quantification, and accuracy were
studied by most authors. Recovery experiments during accuracy evaluation were in general satisfactory,
with usual values between 81 and 109%. However, few methods considered vitamin C stability during
the analytical process, and the study of the precision was not always clear or complete. Potential future
improvements regarding proper method validation are indicated to conclude this review.
Vitamin C is one of the most important vitamins, so reliable information about its content in foodstuffs is
a concern to both consumers and quality control agencies. However, the heterogeneity of food matrixes
and the potential degradation of this vitamin during its analysis create enormous challenges. This review
addresses the development and validation of high-performance liquid chromatography methods for vitamin
C analysis in food commodities, during the period 2000–2014. The main characteristics of vitamin C
are mentioned, along with the strategies adopted by most authors during sample preparation (freezing
and acidification)to avoid vitamin oxidation. After that,the advantages and handicaps of different analytical
methods are discussed. Finally, the main aspects concerning method validation for vitamin C analysis
are critically discussed. Parameters such as selectivity, linearity, limit of quantification, and accuracy were
studied by most authors. Recovery experiments during accuracy evaluation were in general satisfactory,
with usual values between 81 and 109%. However, few methods considered vitamin C stability during
the analytical process, and the study of the precision was not always clear or complete. Potential future
improvements regarding proper method validation are indicated to conclude this review.
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