In PLB proliferation medium, old PLBs were developed into leafy structures from the base of which new PLBs emerged. These leafy structures or shoots were subcultured on ½ MS medium with 2% sucrose, 2 g l-1 peptone, 10% CW and 1 g l-1 activated charcoal for plantlet formation. Banana pulp powder was also used to study its efficacy in root differentiation and growth.