These studies
demonstrated that RSM was effective for investigating the optimum
extraction conditions for producing collagen. There was no
information reported regarding extraction optimization for egg shell
membrane collagen. Therefore, the objective of the present work
was to optimize extraction conditions for pepsin-soluble collagen
(PSC) from eggshell membrane using RSM. This study investigated
the effects of key processing variables (NaOH concentration, alkali
treatment time, enzyme concentration and hydrolysis time) on the
extraction yield of PSC, and determined the optimum conditions
for PSC extraction from eggshell membrane.