The phospholipid composition was determined by 2D thin layer chromatography. The lipid extract was spotted on the TLC (Merck) and phospholipidswere resolved by two-dimensional TLC. The first solvent was chloroform:methanol:water (65:25:4, v/v) and the second chloroform:acetone:methanol:acetic acid:water (50:20:10:10:5, v/v).