In Vitro Growth of Curcuma longa L. in
Response to Five Mineral Elements and Plant
Density in Fed-Batch Culture Systems
Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve
Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch
liquid culture. The experiment had two paired D-optimal designs, testing sucrose fed-batch
and nutrient sucrose fed-batch techniques. When sucrose became depleted, volume was
restored to 5% m/v sucrose in 200 ml of modified liquid MS medium by adding sucrose solutions.
Similarly, nutrient sucrose fed-batch was restored to set points with double concentration
of treatments’ macronutrient and MS micronutrient solutions, along with sucrose
solutions.