In the open-field test, mice are placed in the center of a brightly lit open area and the time spent in the middle, compared with the outer edges, is recorded for 5 minutes. Male GFAP-Cre × flBDNF and CaMKII-Cre × flBDNF KO mice were indistinguishable from their control mice (Figure 5B, D), whereas female conditional KOs of both lines showed a trend for increased time in the middle (Figure 5F, H).