The ability of selected stocks of various mushroom
species to grow well in submerged culture is described
herein. During our studies, a total of 20 species were
grown successfully in submerged culture. All of the
mushroom mycelium cultures were obtained from tissue
culture. The sporophores were collected by the authors
in the general vicinity of this laboratory, with the
exceptions noted in table 1. Using standard aseptic
methods, small pieces of sporophore tissue were placed
on potato-dextrose agar plates. Incubation was at
28 C. Subsequent subculturing produced pure cultures
of mushroom mycelium. Table 1 shows the wide variety
of mushroom species that were grown by submerged
culture in chemically defined medium (Humfeld and
Sugihara, 1949).