The HO-1 stimulation activity was first evaluated at gene level by reverse transcriptase- polymerase chain reaction targeting specific HO-1 gene, and then followed by Western blot in protein level. The in vitro anti-inflammatory effect was measured by quantification of interleukin- 8 (IL-8) level. The in vitro antioxidative activity was measured. Collagen stimulation activity was quantitatively measured by the amount of deposited collagen I in the extracellular matrix.