The various aliquots of the ingesta and the
entire digests were taken into petridishes and were
examined under the microscope for parasites. The
parasites after their recovery from the hosts were
washed in normal saline to free them from mucus.
Then they were fixed in hot 70% alcohol after
fixation the nematode parasites were preserved in
glycerin alcohol (glycerin: 70% alcohol, 1:3) and
were mounted in glycerin and glycerin jelly. Faecal
samples were collected in collection tubes
containing 10% formalin and were examined by
direct smear, flotation and sedimentation techniques
for the presence of eggs (Urquhart et al., 1996). The
drawings of the parasites or parts of parasites were
made with the help of prism type camera lucida and
the measurements were made with the help of
objective (stage) micrometer only and objective and
ocular micrometer. Photographs of the permanent
mounts were taken with the help of Olympus Digital
Camera under Olympus CX21 microscope.
Identification of adult parasites as well as eggs was
done on the basis of various morphological and
morphometric characters (Sahai and Deo, 1964;
Soulsby, 1982).