abstract
Rhodococcus opacus DSM 1069 utilized pine organosolv pretreatment effluent as a sole carbon
and energy source for 120 h at 1.5 w/v% solids concentration and accumulated a
maximum of 26.99 ± 2.88% of its cellular dry weight in oils composed of oleic, palmitic, and
stearic fatty acids. These results establish the potential for lignocellulosic pretreatment
effluent as a feedstock for microbial biodiesel production via oleaginous R. opacus and an
interesting route for biorefinery waste stream optimization.