All reactions were performed in a Mastercycler epGradient thermal cycler (Eppendorf) with the following protocol: initial denaturation for 2min at 94 ◦C, 32 cycles of 1min at 94 ◦C, 1min at 54 ◦C, and 2min at 72 ◦C, with a final
All reactions were performedin a Mastercycler epGradient thermal cycler (Eppendorf) with thefollowing protocol: initial denaturation for 2min at 94 ◦C, 32 cyclesof 1min at 94 ◦C, 1min at 54 ◦C, and 2min at 72 ◦C, with a final