Data also suggested that the use of digital images did not increase intra-examiner reliability in an evident manner,
but the estimated ICC values showed that the protocol that maximized the intra-examiner reproducibility forall examiners was the CP/3C. Although the measurements taken in triplicate were not primordial with regard to
the intra-examiner reliability, when measurements were taken simultaneously by different researchers, the assessment of
more colonies helped increase the accuracy. Thus, taking measurements using a computer or a digital caliper, in triplicate,
seem to be the best protocols, which allow accurate data from different examiners