One gram of leaf sample was homogenized with 2 ml of 0.1 M sodium phosphate buffer (pH 7.0) at 4 C. The homogenate was centrifuged for 20 min at 10,000 rpm.
One gram of leaf sample was homogenized with 2 ml of 0.1 Msodium phosphate buffer (pH 7.0) at 4 C. The homogenate wascentrifuged for 20 min at 10,000 rpm.