This study was conducted during September-October 2009 at two locations in Oregon:1)Salem(Wil- lamette Valley) (44 57 N, 123 02 W) and 2) Herm- iston (north central Oregon) (45 50 N, 119 15 W). In Salem, 12 honey bee colonies from a cooperating beekeeper (Vanderpool Farms) that were approxi- mately equal in size with respect to number of bees and brood area were selected