FDA second experiment was conducted to determine the utility ofFD in maintaining yield while simultaneously improving fineness.Contrasting FD sister lines, CSX2194 and CSX2590, were crossedwith two high micronaire (>4.5) lines CSX5264 and CSX3369 toform four populations. These lines were chosen because theirpedigree which are prone to high micronaire, such as Sicot72 and CSX1303. The high FD parent populations were 10227(CSX2590 × CSX5264) and 10228 (CSX2590 × CSX3369), while thelow FD parent generated populations 10229 (CSX2194 × CSX5264)and 10230 (CSX2194 × CSX3369). These crosses were performed inthe glasshouse; seed from single F2plants were planted in the fieldas F3progeny rows in 2010 with 48 lines per population.