A sample of fresh plant material (300 g) was transferred to a distillation flask containing distilled water (2 L). The flask was attached to a Clevenger apparatus, which was attached to the condenser.Steam distillation continued for 2 h after initial boiling. The organic phase was collected by liquid-liquid extraction of the hydrolate (100 mL) with pentane (30 mL). An excess amount of anhydrous sodium sulfate was added to remove water from the sample, which was then filtered. The filtrate was transferred to the rotary evaporator apparatus (MARCONI, MA-120 series) to acquire the essential o