Fig. 3. Ethidium bromide staining patterns of the PCR products on a 1.5% agarose gel. The arrows indicate the 156 bp of S. mekongi and S. japonicum specific bands. Lane M: DNA size markers (1 kb plus DNA ladder from Invitrogen, Carlsbad, California, USA). Negative control containing no DNA (Lane 1); S. mekongi positive control plasmid (Lane 2); S. japonicum positive control plasmid (Lane 3); S. mekongi-infected Neotricula aperta snails (Lane 4); non-infected N. aperta snails (Lane 5); S. japonicum-infected Oncomelania nosophora snails (Lane 6); non-infected O. nosophora snails (Lane 7); S. mekongi-infected rat feces (Lane 8); negative healthy human feces (Lane 9); and S. japonicum-infected mice feces (Lane 10).