Assay
sensitivity was 0.22 pg ml−1 and the intra- and inter-assay coefficients of variation were 7.1
and 10.1%, respectively. Plasma and oviduct flush samples were analysed for glucose concentrations
using a commercially available kit (Glucose (HK); Sigma Diagnostics, Poole,
Dorset, UK) in combination with glucose standard (GLU GOD-Perid®; Roche Diagnostics
GmbH, Mannheim, Germany). Assay sensitivity was 0.01 mmol l−1 and the intra-assay
coefficient of variation 2.6%. Plasma insulin concentrations were measured by radioimmunoassay
based on the method of Starr et al. (1979) modified according to Armstrong et
al. (2001). Assay sensitivity was 2.1IU l−1 with an intra-assay coefficient of variation of
10.7%.
Assaysensitivity was 0.22 pg ml−1 and the intra- and inter-assay coefficients of variation were 7.1and 10.1%, respectively. Plasma and oviduct flush samples were analysed for glucose concentrationsusing a commercially available kit (Glucose (HK); Sigma Diagnostics, Poole,Dorset, UK) in combination with glucose standard (GLU GOD-Perid®; Roche DiagnosticsGmbH, Mannheim, Germany). Assay sensitivity was 0.01 mmol l−1 and the intra-assaycoefficient of variation 2.6%. Plasma insulin concentrations were measured by radioimmunoassaybased on the method of Starr et al. (1979) modified according to Armstrong etal. (2001). Assay sensitivity was 2.1IU l−1 with an intra-assay coefficient of variation of10.7%.
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