3.2. Effect of triazophos on soluble sugar content and free amino acid content of adult females under eCO2
The three-way ANOVA of the data (Fig. 2) showed that insecticide concentration, rice variety and CO2 concentration significantly influenced the soluble sugar content of adult females (Table 1). The soluble sugar content of adult females increased significantly with the 40 ppm triazophos treatment for both MH63 and TT51 at different DAEs, under both aCO2 or eCO2 (Fig. 2A–C). For the two concentrations of CO2, the grand mean of soluble sugar content of adult females treated with 40 ppm triazophos under eCO2 was significantly higher than under aCO2, increasing by 16.9% and 20.6% at 1 and 3 DAE, respectively. For the two rice varieties, the grand mean of the soluble content of adult females in TT51 treated with 40 ppm triazophos was significantly higher than in MH63, increasing by 27.0% and 29.9%, at 1 and 3 DAE, respectively. Multiple comparisons of the means indicated that the soluble sugar content was significantly higher in the adult females treated with 40 ppm triazophos for both MH63 and TT51 than in untreated controls; the soluble sugar content was significantly higher in the adult females for both MH63 and TT51 under eCO2 than under aCO2.