The Chi-square test revealed no significant difference
( p > 0.05) in the ratio of P. chrysogenum to P. rubens between
locations (Table 3). However, significant differences were
found between multiple locations in the ratios of P. chrysogenum
to other Penicillium, P. rubens to other Penicillium, and
total Chrysogenum complex Penicillium to other Penicillium.
Both the Central and Jubilee Lines were statistically different
to the Outdoors for all three ratio comparisons. The Bakerloo
and Central Line were also significantly different for two ratio
comparisons and borderline significant for the third.
Sequencing of the ITS for 148 Penicilliumisolates revealed that
57 (38.5 %)were similar to Penicilliumbrevicompactum, suggesting
it to be themost common Penicilliumin London. Sequencing also
revealed that 33 (22.3 %) isolateswere similar to P. chrysogenum,
and that 32 (21.6 %) were similar to Penicillium commune.
Fungal genera counts displayed varying proportions of Penicillium
colonies between locations (Fig 5). On the Underground,
the Jubilee Line had the lowest number of Penicilliumcolonies as
a percentage of total fungal colonies with 10.5 %, followed by
the Central Line with 20.1 % and the Bakerloo Line with 32.8 %.
The number of viable fungal conidia inhaled per minute
also varied across locations (Table 4). St Mary’s Hospital had
the fewest conidia with 0.26 conidia per minute whereas the
Underground had an average of 0.98.
Mating-type diagnostics detected MAT1-1 and MAT1-2
isolates in both P. chrysogenum (13:11) and P. rubens (8:14). 1:1
ratios could not be rejected for either species, p ¼ 0.68 and
p ¼ 0.20 respectively.
The Chi-square test revealed no significant difference( p > 0.05) in the ratio of P. chrysogenum to P. rubens betweenlocations (Table 3). However, significant differences werefound between multiple locations in the ratios of P. chrysogenumto other Penicillium, P. rubens to other Penicillium, andtotal Chrysogenum complex Penicillium to other Penicillium.Both the Central and Jubilee Lines were statistically differentto the Outdoors for all three ratio comparisons. The Bakerlooand Central Line were also significantly different for two ratiocomparisons and borderline significant for the third.Sequencing of the ITS for 148 Penicilliumisolates revealed that57 (38.5 %)were similar to Penicilliumbrevicompactum, suggestingit to be themost common Penicilliumin London. Sequencing alsorevealed that 33 (22.3 %) isolateswere similar to P. chrysogenum,and that 32 (21.6 %) were similar to Penicillium commune.Fungal genera counts displayed varying proportions of Penicilliumcolonies between locations (Fig 5). On the Underground,the Jubilee Line had the lowest number of Penicilliumcolonies asa percentage of total fungal colonies with 10.5 %, followed bythe Central Line with 20.1 % and the Bakerloo Line with 32.8 %.The number of viable fungal conidia inhaled per minutealso varied across locations (Table 4). St Mary’s Hospital hadthe fewest conidia with 0.26 conidia per minute whereas theUnderground had an average of 0.98.Mating-type diagnostics detected MAT1-1 and MAT1-2isolates in both P. chrysogenum (13:11) and P. rubens (8:14). 1:1ratios could not be rejected for either species, p ¼ 0.68 andp ¼ 0.20 respectively.
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