Antioxidant Capacity and Total Polyphenols
The free radical scavenging activities using the radical 2,2-
diphenyl-1-picrylhydrazyl method (DPPH) was evaluated
according to that described by Brand-Williams et al.
(1995) with some modifications (Llorach et al. 2004).
Briefly, for each sample, 2 g of arils and 1 g of rind were
homogenized in 5 mL of MeOH/water (80:20 v/v) and then
centrifuged at 15,000 rpm for 20 min. Ten microliters of the
supernatant was mixed with 40 μL of MeOH and added to
950 μL of a 0.094-mM DPPH solution. The absorbance at
515 nm was measured after 50 min of reaction; during this
time, samples were sealed with parafilm to avoid evaporation
losses. The decrease in absorbance was measured in a
Uvikon XS spectrophotometer (Bio-Tek Instrument,
Winooski, VT). Results were expressed as milligrams of
Trolox equivalent 100 g−1