Scanning electron microscopy observations of coffee tissue cultures were made to further document the developmental events occurring during high frequency embryogenesis. Two distinct cell populations of the callus tissue were identified: one consisting of elongated cells and the other of small spherical embryogenic cells. The small spherical cells became separated from one another as well as being segmented from the callus mass as proliferation proceeded. Subsequently, differentiation of the embryogenic cells into globular forms of coffee embryos occurred as the daughter cells of the embryogenic cells remained as a coherent tissue.