On Day 14, one pig in each pen (n = 9),closest to the average body weight for that
pen, was chosen for blood sampling. Blood
samples (approximately 7 mL per sample)
were collected by anterior vena cava puncture
after a 6-hour fast, using plain vacuumfilled
blood collection tubes (21-gauge × 1.5
inches; Serum Clot Activator; Greiner Bioone,
Kremsmunster, Austria). The samples
were then centrifuged (Model VS-5000N;
Vision Scientific, Bucheon, Korea) at 415g
at 4ºC for 15 minutes. Serum was collected
and stored at -25ºC until needed for analysis
of serum parameters.