Documentation and analysis should be based on careful survey of multiple
animals and sites using electron microscopy at low magnifications. These
comparisons should include similarly processed material from control animals.
To document the various nuclear and cytoplasmic features, there
should be electron micrographs taken at magnifications that permit visualization
of nuclear, mitochondrial, endoplasmic reticulum, and plasma membranes
(e.g, 15,000 to 25,000 × ). It is important to realize that both necrotic
and apoptotic cell death and intermediate forms may affect multiple cell
classes besides neurons, particularly glial cells.
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