Antibiotic susceptibilities for DGK-17 were tested by agar over-lay diffusion methodology (Lee et al., 2014). DGK-17 well grown culture (108CFU/ml) was spreaded onto the MRS agar plate and
discs containing specific antibiotics concentration were placed,
further it was incubated overnight at 37 C for 24 h and diameters
of zones inhibition were measured. Antibiotic concentrations were
used in this experiment as follows: Ampicillin (0.5e20mg/ml),
Bacitracin (0.5e150mg/ml), Streptomycin (0.5e300mg/ml), Tetra-cyclin (0e20mg/ml), Vancomycin (0.5e2048mg/ml), Amphotericin
B (0.5e700mg/ml) and Miconazole (0.5e700mg/ml)