Avocado (P. americana) seeds are an un-conventional starch source. The functional and rheological properties of Avocado seed starch suggest applications as an ingredient in food systems and other industrial applications. Use of two different solvents (one containing Tris, NaCl and NaHSO3; the other only sodium bisulphite) in starch isolation had no influence on starch yield or properties. The isolated starches had functional properties similar to those of commercial maize starch. The avocado seed starch had a slightly lower gelatinization temperature (65.7 ◦C) than maize starch (66.32 ◦C) despite their differing amylose contents. Testing of pasting and rheological properties at a 5% (w/v) total solids level showed the avocado seed starches to have enhanced structure at room temperature (25 ◦C). They remained useful at higher temperatures (95 ◦C), with values between 850 and 1000 Pa. Avocado seed starch has potential applications in products such as baby food, sauces, bread products, jellies, candies and sausages. Other possible uses are as a vehicle in pharmaceutical products, and in biodegradable polymers for food packaging