2.7. Lycopene and b-carotene analysis
For carotenoid analysis, four 2 cm2 pericarp sections were cut from the equatorial regions of three tomatoes. The sections from the three tomatoes (twelve sections in total) were combined, weighed and immediately frozen with liquid nitrogen. Samples were then stored at _18 _C, freeze dried, and ground with a mortar and pestle. Samples of tomatoes treated with sun light for 15 days were not taken and analysed. All other samples were prepared and analysed in triplicate.