The stomacher homogenates prepared as described above were
centrifuged (15,000 ×g, 10 min, 10 °C) and the supernatants filtered
through nitrocellulosemembrane filters of 0.2 μmpore size. The filtrate was then diluted (1:5) (v/v) in gelatinphosphate
buffer and kept frozen until analysis, for which the extracts
were thawed overnight at 2 °C.