Preparation Instructions
Preparation Of Bacteria Screening Culture Dishes:
Preparing this product in a concentrated form is not
recommended. The basic steps for preparing culture
medium are the following:
1. Using a container twice the size of the desired
final volume, measure out approximately 90%
of the final required volume of tissue culture
grade water (e.g. Sigma Product No. W3500).
Example: 900 ml for a final volume of 1000 ml.
2. While stirring, add the required amount of
powder.
3. Add additional water to bring the medium to the
final volume.
4. To melt the agar, heat the medium to clarity
(approximately 100°C) while stirring.
5. Sterilize the medium in a validated autoclave at
1 kg/cm2
(15 psi). The medium should attain a
temperature of 121°C for at least 15 min
6. Pour medium into sterile culture dishes or other
vessels as required for your application.
7. The pH of the medium should be approximately
6.9 after autoclaving.