Two media were used: potato dextrose agar medium (PDA)
and maize leaves agar medium (MLA). MLA medium was
made by boiling 30 g fresh maize leaves in 1 l water for
60 min and filtering the suspension through a double layer
of muslin. The volume was made up to 1 l with distilled
water. This medium was specifically chosen because E. turcicum was isolated from fresh maize leaves.