intervention studies, protein supplementation reduced the increases
in IHTG content induced by hypercaloric high-fat and highfructose
diets in healthy subjects [11,12]. However, the longerterm
effects of dietary protein content on IHTG content, thereby
excluding the possible effects of metabolic disturbances and
changes in body weight are unknown. Therefore, in the context
of prevention of metabolic disturbances, the aim of this dietary
intervention study was to assess the effect of a 12-wk iso-energetic
high protein-low carbohydrate (HPLC) diet compared with an isoenergetic
high carbohydrate-low protein (HCLP) diet on IHTG
content in healthy non-obese subjects, at a constant body weight.