The five-cycle repeated batch fermentations using the suspended and TSC-immobilized cultures of S. cerevisiae M30 were carried out in duplicate using blackstrap molasses as the main substrate for ethanol fermentation. The experiment was initiated by transferring the suspended or immobilized cultures into 250 mL of culture medium containing 22–24% (w/v) of initial reducing sugar and 0.5 g/L (NH4)2SO4 in a 500 mL Erlenmeyer flask. The flasks were shaken in the incubator at 150 rpm at 33 °C. The duration of each batch was 48 hours.