The behaviour of S. cerevisiae present in a mixed culture inoculated on the surface of the cheese covered with different films or treated with direct application of antimicrobials is shown in Fig. 2a. The systems containing natamycin (NANI and DA) showed a fungistatic effect for the first 72 h of storage. However, S. cerevisiae resumed its growth, reaching at the end of the storage a count 1.5 log cycles lower than systems without antimicrobials (CNANI and CF). The cheese covered with film NANI or submitted to direct application of natamycin and nisin (DA), presented the same behaviour, indicating that the bioavailability of the antimicrobials present in the film is the same for both treatments.