Colonies of the isolate, suspended in a mixture of TE buffer,SDS (10%; w/v) and proteinase K, were incubated for 1 h at 37 1C. NaCl (5 M) and CTAB/NaCl solution(4.1 g NaCl and 10 g CTAB [N-cetyl-N,N,N,-trimethylammoniumbromide] in pre-warmed 100 mL distilled water) were added and incubated for 10 min at 65 1C.