Sequence comparisons of BPH26 among some rice varieties. Three exon regions of
BPH26 from some rice varieties were amplified by PCR using the genomic DNA as
template. PCR analyses were conducted using KOD-Plus neo (TOYOBO) and the
primers shown in Supplementary Table S2 online. The PCR products were cloned
using Target cloneTM Plus (TOYOBO) and sequenced to compare the alleles from
some rice varieties. In addition to the genomic sequences, cDNA sequences from
ADR52, ASD7, and Norin-PL4 were also used for sequence comparisons. Amino acid
sequences were deduced from these nucleotide sequences.