Shrimp hemolymph was collected from adults and sub-adults, males and females, by inserting a 26G gauge needle attached to a syringe into the ventral sinus, located at the base of the first abdominal segment of P. paulensis. For serum preparation, the hemolymph was transferred to a tube and allowed to coagulate. The clot was stored at 4°C for 24 h, and then repeatedlycentrifugedat 2000 g to remove the serum which was immediately used or aliquoted and stored at - 18°C for further use.
hemocyte lysate supernatant