All bloodstains were prepared on white cloth (100% cotton) which was cut into squares of approximately 1 cm × 1 cm, previously autoclaved. A 2 μL drop of venous blood (of the same donor for all times analyzed) was added to the squares of cloth and allowed to dry for 48 h. The blood was not subject to any form of anticoagulant. All samples were stored at room temperature, to simulate conditions found in crime scenes. Controls were composed of bloodstains without application of any reagent. For the immunological tests, sample size (n) was 20. For the DNA tests, n varied between 13 and 29.