This corresponded to a cell density of
approx. 2.2 log (CFU/mL). Next, every well of the microtiter plate
was inoculated with 10 mL of the bacterial suspension, resulting in
approx. 1 to 2 individual cells per well. In order to obtain surface
colonies, the spot inoculation method was applied, i.e., the 10 mL
inoculum was pipetted as a single drop onto the agar surface. The
microtiter plate was placed in an incubator at 30 C without the lid.
After 30 min, the liquid inoculum had dried completely onto the
agar surface, and the microtiter plate was placed in a temperaturecontrolled
microplate reader at 30 C.
ของสายพันธุ์ที่2. ถูกเจือจางใน BHI ของเหลว6ครั้ง