2.5. Lactic and acetic acid analyses
The incubated samples at 24, 48, and 72 h were centrifuged at
13,000 rpm for 10 min to remove all insoluble particles and the
lactic and acetic acids fermentation products were quantified using
a BioRad HPX-87H HPLC column (Watford, UK) at 50 C, with
a 0.005 mM H2SO4 as the mobile phase, in isocratic mode, at a flow
rate of 0.6 mL min1 (Sanz et al., 2005). The analyses were carried
out in triplicate.
Since minor levels of acetic acid were initially present in the
MRS broth, this value was quantified and subtracted from the
amounts calculated for the samples subjected to incubation.
2.5. Lactic and acetic acid analysesThe incubated samples at 24, 48, and 72 h were centrifuged at13,000 rpm for 10 min to remove all insoluble particles and thelactic and acetic acids fermentation products were quantified usinga BioRad HPX-87H HPLC column (Watford, UK) at 50 C, witha 0.005 mM H2SO4 as the mobile phase, in isocratic mode, at a flowrate of 0.6 mL min1 (Sanz et al., 2005). The analyses were carriedout in triplicate.Since minor levels of acetic acid were initially present in theMRS broth, this value was quantified and subtracted from theamounts calculated for the samples subjected to incubation.
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