The sperm-to-extender ratio was 1:6 (v/v) and the volumes of extended sperm samples prepared were 0.5, 1.0, 2.0 and 4.0 ml. The chemical composition of each extender is shown in Table 1.
The sperm-to-extender ratio was 1:6 (v/v) and the volumes of extended sperm samplesprepared were 0.5, 1.0, 2.0 and 4.0 ml. The chemical composition of each extender is shown in Table 1.