1 g of defatted flowers or leaves were taken and shifted into round bottom flask(100 ml capacity)containing 50 ml aqueous ethanol.After fixing a condenser,it was left for reflux condensation on heating mantel (Isopad LG2/ER 100 ml,model 913323,Tmax-450c)for two and half hours.Extract was filtered and diluted up to 50 ml.