The copper accumulation in both shoots and roots increased with the increase in the external cu level(Figure 2). The accumulation of copper in roots was higher than in shoots in all the treatments(Figure 2). The maxi mum Cu content was observed in both roots(73.6 mg kg i DW and shoots(28.7 mg kg DW at the highest level of Cu(5.0 mg dm CuSO, x 5H O). followed by 33.4 and 15.0 mg cu kg DW of roots and shoots, respectively, at 2.5 mg CuSO. x 5HaO dm of the MS medium, and 14.6 and 8.19 mg Cu kg DW of roots and shoots, respectively, at 1.25 mg Cuso x Hao dm 3 of the MS medium, and 7.8 and 4.45 mg cu kg DW of roots and shoots, respecti vely, at 0.625 mg CuSO. x 5Hao dm 3 of the MS medium The iron(Fe) content in roots tended to increase with the increasing cu concentration in the growth medium, while just slightly rising in shoots(Figure 3). The iron concentration increased by up to 72% (228 to 391 mg Fe kg Dw rom 0.025 to 5.0 mg CuS0, x 5H O dm of MS medium) in roots and 40% (76.5 to 107.0 mg Fe kg Dw from 0.025 to 5.0 mg Cuso,x 5HLO dma of MS medium in shoots(Figure 3) The zinc(Zn) content in roots and shoots showed a decreasing trend with the increasing cu in the growth medium(Figure 4). The zinc