bacteriaMarine sediment samples were collected from the ChineseBohai Sea (123◦371E, 39◦6972N) at a depth less than 100 m andstored at −20◦C until use.
For screening of chitinolytic bacteria, allisolates were incubated on Bacto Marine Broth agar 2216E (Difco)containing 0.5% colloidal chitin [12] at 15◦C.