A 6% (w/v) fish gelatin solution was prepared in distilled water under continuous stirring and heating at 70 °C during 30 min. Glycerol (10% w/w of polymer dry matter) was added to the film solution after dispersion of gelatin. pH of gelatin solution was adjusted to about 4.9. The film-forming solution was then poured into a plastic Petri dish (13.5 cm diameter). In order to produce the films, the solvent was removed by drying in a ventilated climatic chamber (KBF 240 Binder, ODIL, France) at 25 °C and 45% relative humidity (for 18–24 h depending on the volume of film-forming solution poured). After drying, the films were peeled off from the surface and stored up to equilibration in a ventilated climatic chamber (KBF 240 Binder, ODIL, and France) at 50% RH and 25 °C before each measurement.