Rhizosphere region are the most nutrient rich niche among the soil
environment that feeds the soil dwelling microorganisms. Totally eight isolates
were obtained from the rhizosphere of sugarcane plant that were grown in
agricultural fields of Vellore district. The study was further preceded for in vitro
screening of plant growth promoting traits viz., production of Indole acetic acid
(IAA), Hydrogen cyanide, Siderophore, solubilization of phosphate, N fixation
and 1-aminocyclopropane-1- carboxylic acid (ACC) deaminase activity of the
isolates. Siderophore and phosphate solubilization were screened qualitatively on
chrome azurol S and Pikovskaya agar. All the isolates exhibited positively for
Tri-calcium phosphate solubilisation. The results showed that all isolates
produced IAA ranging from 9.14 ± 0.27 to 32.32±0.98 μmol ml-1 in the presence
of L-Tryptophan. Five isolates displayed siderophore production and none of the
isolates turned positive for Hydrogen cyanide production. Only three isolates
were able to cleave 1-aminocyclopropane-1-carboxylate. The bacterial isolates
showed difference in plant growth promotion traits while the isolate Vsc-14
exhibited multiple plant growth promotion activity.