The cross-streaking method is a novel detection method that was developed from the results of the evaluation of the methods described above and is proposed as an alternative to the application of PCR for confirmation of colony identification. Samples from TT broth were initially streaked to XLT4, and presumptive positive colonies were subsequently restreaked to CPS agar.The cross-streaking method classifies a colony as positive if it exhibits typical Salmonella appearance on both XLT4
and CSP agars and it classifies a colony as negative if it does not. In order to ensure isolation of a single colony from primary XLT4 agar isolation, colonies were also re-streaked to XLT4.