Soybean waste is by product in production of tofu and soymilk. Soybean waste commonly dumps directly to the water sewer and made environmental problems like eutrotification. Soybean waste contains 23% hemicellulose, 16% cellulose dan 28% protein. Xylan rich hemicellulose is potential source of xylooligosacharides (XOS). XOS was known as functional food with prebiotic activities. In this research, xylan was isolated from soybean waste then hydrolyzed by endoxylanase enzyme of Bacillus sp from soil termite abdominal. At the beginning, Soybean waste was processed by reflux to exclude lipid compound. Then process continued by alkaline treatment using NaOH 4-18% to separate the xylan from lignocellulose complex. Isolated xylan was hydrolyzed by endo-β-1,4-D-xylanase to get XOS. Hydrolised product analysed by TLC and HPLC. TLC analysis shown thick spot confirmed as xylopentaose (X5) compare to the Rf of the standard compound. HPLC data supported these analyses with the result of X5 as highest component (6959,88 ppm) beside the other XOS such as xylobiosa (X2) 6,34 ppm and xylosa (X1) 7,1 ppm. With the same enzyme, the result of hydrolyzed of soybean wastes xylan is not different with hydrolysed of oat spelt xylan, the common xylan source.
extraction time was 6 hrs which giving the maximum yield (based on total solid) of 29.5%. The
optimal condition of pectic oligosaccharide reaction were pH of 4 and 50 oC which gave reducing
sugar of 3.54 mg/ml and. The chemical composition analyzed by HPLC confirmed that pectic
oligosaccharide consisted of mono galacturonic acid, di galacturonic acid and tri galacturonic acid.
Particularly, tri galacturonic acid has potential use as prebiotic. The result also showed that pectic
oligosaccharides could promote the growth of probiotic (Lactobacillus plantarum) which confirmed
their prebiotic property.
Keywords:
Soybean waste is by product in production of tofu and soymilk. Soybean waste commonly dumps directly to the water sewer and made environmental problems like eutrotification. Soybean waste contains 23% hemicellulose, 16% cellulose dan 28% protein. Xylan rich hemicellulose is potential source of xylooligosacharides (XOS). XOS was known as functional food with prebiotic activities. In this research, xylan was isolated from soybean waste then hydrolyzed by endoxylanase enzyme of Bacillus sp from soil termite abdominal. At the beginning, Soybean waste was processed by reflux to exclude lipid compound. Then process continued by alkaline treatment using NaOH 4-18% to separate the xylan from lignocellulose complex. Isolated xylan was hydrolyzed by endo-β-1,4-D-xylanase to get XOS. Hydrolised product analysed by TLC and HPLC. TLC analysis shown thick spot confirmed as xylopentaose (X5) compare to the Rf of the standard compound. HPLC data supported these analyses with the result of X5 as highest component (6959,88 ppm) beside the other XOS such as xylobiosa (X2) 6,34 ppm and xylosa (X1) 7,1 ppm. With the same enzyme, the result of hydrolyzed of soybean wastes xylan is not different with hydrolysed of oat spelt xylan, the common xylan source.extraction time was 6 hrs which giving the maximum yield (based on total solid) of 29.5%. Theoptimal condition of pectic oligosaccharide reaction were pH of 4 and 50 oC which gave reducingsugar of 3.54 mg/ml and. The chemical composition analyzed by HPLC confirmed that pecticoligosaccharide consisted of mono galacturonic acid, di galacturonic acid and tri galacturonic acid.Particularly, tri galacturonic acid has potential use as prebiotic. The result also showed that pecticoligosaccharides could promote the growth of probiotic (Lactobacillus plantarum) which confirmedtheir prebiotic property.Keywords:
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