Methane production from llama and cow manures from the Bolivian high plateau (The “Altiplano”) was studied using a parallel reactor set-up consisting of 10 lab-scale biogasifiers. The effects of pressure (495 and 760 mmHg), temperature (11 and View the MathML source), hydraulic retention time (20 and 50 days), and manure content in the slurry (10%, 20% and 50%) were evaluated with respect to productivity and methane yields based on two 24-1 fractional factorial designs with 8 treatments for each kind of manure. The reactors were operated semi-continuously with daily manure feeding for periods between 50 and 100 days. Temperature was the main factor effect found, and the hydraulic retention time and the manure content in feed were also found significant whereas the effect of pressure was not significant in the range studied. The methane yield obtained with cow manure at View the MathML source was between 6.4 and View the MathML source (volatile solids added) whereas at View the MathML source the methane yield was between 49.6 and View the MathML source. The methane yield from llama manure was somewhat lower than for cow manure (between 3.3 and View the MathML source at View the MathML source and between 35.6 and View the MathML source at View the MathML source, respectively). However, overall llama manure was found to be the best raw material of the two for biogas production, due to its high content of volatile solid—higher than has been previously reported for most manures—and also its high nitrogen and phosphorous content.